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A core facility for the Hanson Institute was established in 2002 following the acquisition of a new confocal microscope and a high-speed sorting flow cytometer.
The Detmold Family Trust Cell Imaging Centre was so named to acknowledge the Detmold family's generous donation towards the purchase of the flow cytometer. The gift commemorates Mr Phillip Detmold, who died of leukaemia in 2001. These funds were met by an equal contribution from the Department of Human Services.
Funding for the new confocal microscope came predominantly from the Wellcome Trust and was due to the tireless efforts of a small group of researchers at the Hanson Institute. The Facility provides specialist cell-imaging which enhances the research activities being undertaken across the campus.
The Center has recently installed a second high speed cell sorter in a collaborative arrangement with the University of Adelaide, and a second UV capable confocal microscope will also be installed by Mid 2005. In addition to this, two flow cytometric analysers are housed in the centre.
The role of the Facility is to provide staff and students of the IMVS/Hanson Institute with a high-quality flow-cytometry and cell-imaging service, where specialist operators can assist staff members and students in getting the very best from their studies. In addition to providing a valuable resource and service, staff of the Facility will develop new techniques to gain the maximum benefit from these specialised instruments.
Flow Cytometry
The facility currently has two Coulter XL flow cytometric analysers, and Becton-Dickinson ARIA and a Coulter ALTRA flow cytometric sorters.
Imaging
The facility has recently commissioned a BioRad Radiance 2100 Confocal Microscope. This instrument is equipped with three lasers (Argon; 457,476,488,514nm; Green HeNe; 543nm; Red Diode; 637nm). The instrument can collect from three PMT's as well as transmitted light, allowing the analysis of a wide range of fluorochromes. A new Nikon C1 laser scanning confocal microscope with near UV laser capability is expected to be installed and operational by the middle of 2005. This new system is equipped with three solid lasers (Radius 405nm; Coherent 488 Sapphire laser, Coherent Compass 215 M-10 /532nm). The C1 system can collect image from three PMT’s as well as high contrast Phase and DIC transmission and scans at a high 500 lines/sec, allowing high-speed image capture.
Bookings
The facility has a networked booking system. Please contact Alan Bishop for further information and access.
Contacts
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